Journal: Cells

Article Title: Understanding Cell Model Characteristics—RNA Expression Profiling in Primary and Immortalized Human Mesothelial Cells, and in Human Vein and Microvascular Endothelial Cells

doi: 10.3390/cells11193133

Figure Lengend Snippet: Identified protein-coding transcripts and their presence in studied cell lines. From a total of 12,760, 9853 transcripts were shared between all cell lines, 366, 631, 99 and 87 transcripts were HPMC-, MeT-5A-, HUVEC- and 87 HCMEC-specific, respectively.

Article Snippet: Human endothelial umbilical vein cells (HUVEC), human cardiac microvascular endothelial cells (HCMEC) and the immortalized mesothelial cell line (MeT-5A) were purchased from established vendors (HUVEC and HCMEC from Promocell, Heidelberg, Germany; MeT-5A (ATCC ® CRL-9444™) from LGC Standards, Wesel, Germany).

Techniques:

Journal: Cells

Article Title: Understanding Cell Model Characteristics—RNA Expression Profiling in Primary and Immortalized Human Mesothelial Cells, and in Human Vein and Microvascular Endothelial Cells

doi: 10.3390/cells11193133

Figure Lengend Snippet: Mesothelial cell model comparisons ( A ) Venn diagram of differentially expressed genes in human primary peritoneal mesothelial cells (HPMC) versus immortalized pleural mesothelial cell line (MeT-5A). ( B ) Gene Ontology (GO) term enrichment analysis of 771 HPMC-specific genes. Number of genes per GO term and fold enrichment are given by bubble size and on the x -axis, respectively. The pink categories represent significantly regulated biological processes (BP), the light-yellow categories cellular components (CC), the purple categories blue molecular function (MF), as identified by Panther Gene Ontology Enrichment Analysis (FDR Q-value < 0.05). ( C ) 944 MeT-5A-specific genes ( D ) Volcano plot of differentially regulated transcripts in HPMC vs. MeT-5A (FDR Q-value < 0.05, abs(log2FC) > 2), 488 genes were less and 612 were more abundant in HPMC.

Article Snippet: Human endothelial umbilical vein cells (HUVEC), human cardiac microvascular endothelial cells (HCMEC) and the immortalized mesothelial cell line (MeT-5A) were purchased from established vendors (HUVEC and HCMEC from Promocell, Heidelberg, Germany; MeT-5A (ATCC ® CRL-9444™) from LGC Standards, Wesel, Germany).

Techniques:

Journal: Cells

Article Title: Understanding Cell Model Characteristics—RNA Expression Profiling in Primary and Immortalized Human Mesothelial Cells, and in Human Vein and Microvascular Endothelial Cells

doi: 10.3390/cells11193133

Figure Lengend Snippet: Gene set enrichment analysis (GSEA) of differentially expressed genes (DEGs) in mesothelial cells. Of the 10,624 genes shared by HPMC and MeT-5A, significantly different regulation (FDR Q-value < 0.05) of gene sets as identified by GSEA are shown. Nodes of GO terms of more abundant gene sets are represented in red, and of less abundant gene sets in blue. Node size reflects the gene set size and letter size indicates the size of the summary term. Genes shared by gene sets are reflected by connecting lines.

Article Snippet: Human endothelial umbilical vein cells (HUVEC), human cardiac microvascular endothelial cells (HCMEC) and the immortalized mesothelial cell line (MeT-5A) were purchased from established vendors (HUVEC and HCMEC from Promocell, Heidelberg, Germany; MeT-5A (ATCC ® CRL-9444™) from LGC Standards, Wesel, Germany).

Techniques: